Recent Representative Publications from the Huberman Lab

Zhu, J., Newlon, C.S., and Huberman, J.A. (1992) Localization of a DNA Replication Origin and Termination Zone on Chromosome III of Saccharomyces cerevisiae. Mol. Cell. Biol. 12: 4733-4741. Medline

Zhu, J., Brun, C., Kurooka, H., Yanagida, M., and Huberman, J.A. (1992) Identification and characterization of a complex chromosomal replication origin in Schizosaccharomyces pombe. Chromosoma 102: S7-S16. Medline

Burhans, W.C. and Huberman, J.A. (1994) DNA replication origins in animal cells: a question of context? Science 263: 639-640 (minireview). PDF

Dubey, D.D., Zhu, J., Carlson, D.L., Sharma, K. and Huberman, J.A. (1994) Three ARS elements contribute to the ura4 replication origin region in the fission yeast, Schizosaccharomyces pombe. EMBO J. 13: 3638-3647. Medline

Zhu, J., Carlson, D.L., Dubey, D.D., Sharma, K. and Huberman, J.A. (1994) Comparison of the two major ARS elements of the URA4 replication origin region with other ARS elements in the fission yeast, Schizosaccharomyces pombe. Chromosoma 103: 414-422. Medline

Yoon, Y., Sanchez, J.A., Brun, C., and Huberman, J.A. (1995) Mapping of replication initiation sites in human ribosomal DNA by nascent strand abundance analysis. Mol. Cell. Biol. 15: 2482-2489, 1995. PDF

Huberman, J.A. (1995) Importance of measuring nucleic acid absorbance at 240 nm as well as at 260 and 280 nm. BioTechniques 18: 636 (brief letter).

Brun, C.,Brun, C., Dijkwel, P.A., Little, R.D., Hamlin, J.L., Schildkraut, C.L., and Huberman, J.A. (1995) Yeast and mammalian replication intermediates migrate similarly in two-dimensional gels. Chromosoma 104:92-102. Medline

Brun, C., Dubey, D.D., and Huberman, J.A. (1995) pDblet, a stable autonomously replicating shuttle vector for Schizosaccharomyces pombe. Gene 164:173-177. Medline

Huberman, J.A. (1995) Cell cycle: a licence to replicate. Nature 375: 360-361 (invited minireview).

Huberman, J.A. (1995) Prokaryotic and eukaryotic replicons. Cell 82: 535-542 (invited meeting summary).

Murakami, Y., Huberman, J.A., and Hurwitz, J. (1996) Identification, purification and molecular cloning of autonomously replicating sequence-binding protein 1 from fission yeast Schizosaccharomyces pombe. Proc. Natl. Acad. Sci. 93: 502-507. PDF

Givens, R.M., Saavedra, R.A., and Huberman, J.A. (1996) Topological complexity of SV40 minichromosomes. J. Mol. Biol 257: 53-65. PDF

Dubey, D.D., Kim, S.M., Todorov, I. and Huberman, J.A. (1996) Large, complex modular structure of a fission yeast DNA replication origin. Current Biology 6: 467-473. Medline

Huberman, J.A. (1996) Cell-cycle control of S phase: a comparison of two yeasts. Chromosoma 105: 197-203 (Focus review). PDF

Vernis, L., Abbas, A., Chasles, M. Gaillardin, C. M., Brun, C., Huberman, J.A. and Fournier, P. (1997) An origin of replication and a centromere are both needed to establish a replicative plasmid in the yeast Yarrowia lipolytica. Mol. Cell. Biol. 17: 1995-2004. PDF

Lee, J.-K., Huberman, J.A. and Hurwitz, J. (1997) Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe. Proc. Natl. Acad. Sci. USA 94: 8427-8432. PDF

Huberman, J.A. (1997) Mapping replication origins, pause sites and termini by neutral/alkaline two-dimensional gel electrophoresis. Methods: A Companion to Methods in Enzymology 13: 247-257 (invited methods review). Medline

Sanchez, J.A., Kim, S.-M., and Huberman, J.A. (1998) Ribosomal DNA replication in the fission yeast, Schizosaccharomyces pombe. Exptl. Cell Res. 238: 220-230. Medline

Todorov, I.T., Werness, B.A., Wang, H.-Q., Buddharaju, L.N., Todorova, P.D., Slocum, H.K., Brooks, J.S.J. and Huberman, J.A. (1998) HsMCM2/BM28, a novel proliferation marker for human tumors and normal tissues. Laboratory Investigation 78: 73-78. PDF

Sanchez, J.P., Murakami, Y. Huberman, J.A. and Hurwitz, J. (1998) Isolation, characterization and molecular cloning of a protein (Abp2) that binds to a Schizosaccharomyces pombe Origin of Replication (ars3002). Mol. Cell. Biol. 18: 1670-1681. PDF

Huberman, J.A. (1998) Choosing a place to begin. Science 281: 929-930 (invited minireview). Full text online.

Kim, S.-M. and Huberman, J.A. (1998) Multiple orientation-dependent, synergistically interacting, similar domains in the ribosomal DNA replication origin of the fission yeast, Schizosaccharomyces pombe. Mol. Cell. Biol. 18: 7294-7303. Full text online.

Huberman, J.A. (1999) DNA damage and replication checkpoints in the fission yeast, Schizosaccharomyces pombe. Progr. Nucl. Acids Res. Mol. Biol. 62: 369-395 (invited review). Medline.

Huberman, J.A. (1999) Genetic methods for characterizing the cis-acting components of yeast DNA replication origins. Methods: A Companion to Methods in Enzymology 18: 356-367 (invited review). Medline.

Kim, S. -M. and Huberman, J.A. (1999) Influence of a replication enhancer on the hierarchy of origin efficiencies within a cluster of DNA replication origins. J. Mol. Biol. 288: 867-882. PDF.

Santocanale, C., Sharma, K. and Diffley, J.F.X. (1999) Activation of dormant origins of DNA replication in budding yeast. Genes Dev. 13: 2360-2364. This paper describes the results of a collaboration between Karuna Sharma, in the Huberman laboratory, and Corrado Santocanale and John Diffley at the ICRF Clare Hall Laboratories in England. Freely available at

Weinberger, M., Trabold, P.A., Lu, M., Sharma, K., Huberman, J.A. and Burhans, W.C. (1999) Induction by adozelesin and hydroxyurea of origin recognition complex-dependent DNA damage and DNA replication checkpoints in Saccharomyces cerevisiae. J. Biol. Chem. 274: 35975-35984. Freely available at

Berezney, R., Dubey, D.D. and Huberman, J.A. (2000) Heterogeneity of eukaryotic replicons, replicon clusters, and replication foci. Chromosoma 108: 471-484 (review). PDF

Sharma, K., Weinberger, M. and Huberman, J.A. (2001) Roles for internal and flanking sequences in regulating the activity of mating-type-silencer-associated replication origins in Saccharomyces cerevisiae. Genetics 159: 35-45. Freely available from Genetics web site.

Kim, S.-M., Zhang, D.-Y. and Huberman, J.A. (2001) Multiple redundant sequence elements within the fission yeast ura4 replication origin enhancer. BMC Molecular Biology 2: 1. Freely available at

D.-F. Tan, J.A. Huberman, A. Hyland, G. Loewen, J.S.J. Brooks, A. Beck, H.K. Slocum, N. Ramnath, I.T. Todorov and G. Bepler. (2001) HsMCM2-A promising marker For premalignant lesions of the lung: a cohort study. BMC Cancer 1: 6. Freely available at

Kim, S.-M. and Huberman, J.A. (2001) Regulation of replication timing in fission yeast. EMBO J., 20:6115-6126. PDF

Ramnath, N., Hernandez, F.J., Tan, D.-F., Huberman, J.A.., Natarajan, N., Beck, A.F., Hyland, A., Todorov, I.T., Brooks, J.S.J., and Bepler, G. (2001) MCM2 is an independent predictor of survival in patients with non-small-cell lung cancer. J. Clin. Oncol. 19: 4259-4266, 2001. Read abstract and download full text from JCO web site. The abstract is free but the article isn't. If you're not a subscriber to JCO, you can send me an e-mail request for a PDF reprint.

Marchetti, M.A., Kumar, S., Hartsuiker, E., Maftahi, M., Carr, A.M., Freyer, G.A., Burhans, W.C. and Huberman, J.A. (2002) A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway. Proc. Natl. Acad. Sci, USA 99:7472-7477. Freely available from the PNAS web site.

Kim, S.-M., Dubey, D.D. and Huberman, J.A. (2003) Early-replicating heterochromatin. Genes Dev. 17: 330-335. PDF

Huberman, J.A. (2003) My unusually dedicated mentor. Biophys. Chem. 100: 95-97. (Recollections of my outstanding undergraduate mentor, John Tileston Edsall). PDF

Antunes, D.F., Kim, S.-M., Huberman, J.A. and de Morais, M.A. (2003) Motifs in Schizosaccharomyces pombe ars3002 important for replication origin activity in Saccharomyces cerevisiae. Plasmid 50: 113-119. PDF

Masukata, H., Huberman, J.A., Frattini, M.G. and Kelly, T.J. (2003) DNA replication in S. pombe. In "The Molecular Biology of Schizosaccharomyces pombe", ed. R. Egel, Pp. 73-99. Springer-Verlag, Heidelberg (review).

Bowmaker, M., Ming, Y.Y., Yasukawa, T., Reyes, A., Jacobs, H.T., Huberman, J.A., and Holt, I.J. (2003) Mammalian mitochondrial DNA replicates bidirectionally from an initiation zone. J. Biol. Chem. 278: 50961-50969. PDF

Huberman, J.A. (2003) DNA replication, the cell cycle, and genome stability. Mutation Research 532: 1-4 (editorial). PDF

Burhans, W.C., Weinberger, M., Marchetti, M.A., Ramachandran, L., D'Urso, G., and Huberman, J.A. (2003) Apoptosis-like yeast cell death in response to DNA damage and replication defects. Mutation Research 532: 227-243 (review). PDF

Yompakdee, C. and Huberman, J.A. (2004) Enforcement of late replication origin firing by clusters of short G-rich DNA sequences. J Biol. Chem. 279: 42337-42344. PDF

Kumar, S. and Huberman, J.A. (2004) On the slowing of S phase in response to DNA damage in fission yeast. J. Biol. Chem. 279: 43574-43580. PDF

Sommariva, S., Pellny, T.K., Karahan, N., Kumar, S., Huberman, J.A. and Dalgaard, J.Z. (2005) Schizosaccharomyces pombe Swi1p, Swi3p and Hsk1p are components of a novel S-phase response pathway to alkylation damage. Mol. Cell. Biol. 25:2770-2784. PDF

Weinberger, M., Ramachandran, L., Feng, L., Sharma, K., Sun, X., Marchetti, M., Huberman, J.A. and Burhans, W.C. (2005) Apoptosis in budding yeast caused by defects in initiation of DNA replication. J. Cell Sci. 118:3543-3553. PDF

Marchetti, M.A., Weinberger, M., Murakami, Y., Burhans, W.C., and Huberman, J.A. (2006) Production of reactive oxygen species in response to replication stress and inappropriate mitosis in fission yeast. J. Cell Sci. 119:124-131. PDF

Foiani, M., Kumar, S. and Huberman, J.A. (2006) Responses to aberrant replication and DNA damage in yeasts. In "DNA Replication and Human Disease", DePamphilis, M.L., editor. Pp. 335-356. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. (Review).

Yang, J., Ramnath, N., Moysich, K.B., Asch, H.L., Swede, H., Alrawi, S.J., Huberman, J., Geradts, J., Brooks, J.S.J. and Tan, D. (2006) Prognostic significance of MCM2, Ki-67 and gelsolin in non-small cell lung cancer. BMC Cancer 6: 203. Freely available at

This page was updated August 25, 2006.

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