Answer to question 1 from 1994
The sources suggested in class from which you might obtain help are (i) cloning manuals, (ii) methods journals such as BioTechniques, (iii) the BioNet.MolBio.Methds-Reagnts newsgroup, (iv) manufacturers' catalogs, (v) general journals covering molecular biological topics, and (vi) colleagues. In addition, some of you pointed out in your answers that literature searching tools, such as Hubnet (which permits searching the Medline data base) are also useful sources of information. With regards to this specific question, many of you suggested that the triple-stranded vector or a ligase chain reaction kit might be available from a manufacturer who could be contacted by telephone. Even if not commercially available, each of these was developed by a scientist who reported her/his observations in the literature. That scientist could be contacted by telephone or other means. Paramecium is a single-celled eukaryotic microorganism that has been a favorite experimental organism for more than 100 years, so there is a great deal of literature on it. Since Paramecium has virtually no medical relevance, however, that literature would have to be searched in a different database than Medline. Biological Abstracts would be a good starting point.
Return to Molecular Methods page