BIR572 -- Regulatory Mechanisms of Eukaryotic Cells

Notes for Lectures on Yeast Genetics

March 20 -- March 27, 2007

This page was last updated March 20, 2007

Joel Huberman
Dept. of Cancer Biology
C&V 501
Extension 3047
Preferred e-mail address:

Cerevisiae and Pombe

Saccharomyces cerevisiae and Schizosaccharomyces pombe represent two very different types of yeast. Molecular evidence suggests that they diverged over 1 billion years ago. S. cerevisiae has "evolved" more rapidly than S. pombe since divergence.

In some ways, S. pombe resembles animal cells more than does S. cerevisiae (e.g. division pattern; centromeres; introns; heterochromatin), but in other respects S. cerevisiae is more similar to animal cells (e.g. relative lengths of G1 and G2; synaptonemal complexes).

Reasons why yeasts are excellent eukaryotic model organisms
Fig. 1. Size and appearance of cells

Fig. 1

Life cycles
Cell cycles

Fig. 5

Extrachromosomal elements
Genetic nomenclature
Making mutants
Random spore analysis (a poor or clumsy person's alternative to tetrad analysis)

Mapping by tetrad analysis

Modern mapping procedures
Other modern techniques (just a few examples from many possibilities)

Here are some review aids. Clicking on the links below will cause a Microsoft Word Document to be downloaded to your hard drive, or to be opened in Word on your computer, depending on the settings in your web browser.
Exam questions from 2001
Exam answers from 2001
Exam questions from 2002
Exam answers from 2002
Exam questions from 2003
Exam answers from 2003
Exam questions from 2004
Exam answers from 2004
Exam questions from 2005
Exam answers from 2005
Exam questions from 2006
Exam answers from 2006

References (where to find more)

Web References


Papers for class discussion

Supplementary papers (and papers assigned for discussion in previous classes)