The raw video for these movies was filmed by Dr. Robert Givens and me in late 2007 and early 2008, but the video wasn't converted by me into finalized movies until August, 2012. Dr. Givens and I hope that these movies will prove useful to the yeast research community.
The movies demonstrate some of the techniques described verbally in our 2011 publication (RM Givens, LD Mesner, JL Hamlin, MJ Buck and JA Huberman, Integrity of chromatin and replicating DNA in nuclei released from fission yeast by semi-automated grinding in liquid nitrogen. BMC Research Notes 2011, 4:499). The publication is available for free at http://www.biomedcentral.com/1756-0500/4/499. Although these techniques were developed for fission yeast cells, they should be applicable with only minor modifications to budding yeast cells and to any other organism having tough cell walls.
Each of the movies is available from YouTube (see links below), and each is also available in .m4v format as a download from this website (see links below). The .m4v format will be useful to people who may wish to view the movie from a location not having Internet access. Note that the .m4v files are available in two forms: (1) native .m4v, and (2) compressed (zipped) .m4v. The compressed files are only slightly smaller than the non-compressed files. All are large and while require minutes to hours to download, depending on the speed of your Internet connection.
If you have questions about, or problems with, any of these movies, please send me an e-mail (huberman AT buffalo DOT edu).